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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which leads to chromosome instability, and thus may lead to tumorigenesis. This protein was identified to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important function in advertising and marketing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two additional splice variants ended up documented but comprehensive nucleotide sequence information and facts will not be nonetheless offered.
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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide commonly is made up of the epitope acknowledged because of the antibody.
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This manual outlines further more achievable optimization of mobile and immunoassay parameters to be certain the very best results are...
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This manual outlines even more feasible optimization of cellular and immunoassay parameters to be certain the absolute best success are...
Antibodies sure to the blocking peptide not bind to the epitope about the concentrate on protein. By comparing the staining within the blocked antibody versus the antibody on your own, one can see which staining is particular.
Goal Details Cyclin E belongs to your highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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